A simple method for the purification of human peripheral blood monocytes. A substitute for Sepracell-MN.
نویسندگان
چکیده
Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient which matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 x 10(6) monocytes with a purity of 83% were obtained from 30-40 ml of blood. These monocytes were at least 97% viable and responded to chemotactic stimuli in comparable numbers to those prepared using Sepracell-MN.
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ورودعنوان ژورنال:
- Journal of immunological methods
دوره 144 2 شماره
صفحات -
تاریخ انتشار 1991